Which Lane Contains The Dna Fragments Produced By Digestion Of The Plasmid With Enzymes X And Y?

Which two enzymes should be used to cleave the vector and the chromosomal DNA to produce complementary ends that will lead to this result?

Which two enzymes should be used to cleave the vector and the chromosomal DNA to produce complementary ends that will lead to this result? You would like to ligate a fragment you cleaved with the restriction enzyme BamHI into a plasmid you cleaved with a different enzyme.

What is the function of the enzyme DNA polymerase ls7a?

a class of enzyme that recognizes specific, short nucleotide sequences in double-stranded DNA and cleaves the DNA at these sites. You just studied 102 terms!

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What is the name of the class of enzymes that recognizes and cuts a specific sequence of DNA quizlet?

What is the name of the class of enzymes that recognizes and cuts a specific sequence of DNA? Restriction enzymes recognize certain DNA sequences and: some of them will cut straight through, while others will leave an overhang at both ends of the cut.

Which Strand top or bottom is the leading strand template?

The bottom strand will be the template for leading strand synthesis.

How do you make a plasmid DNA?

The basic steps are:

  1. Cut open the plasmid and “paste” in the gene. This process relies on restriction enzymes (which cut DNA) and DNA ligase (which joins DNA).
  2. Insert the plasmid into bacteria.
  3. Grow up lots of plasmid-carrying bacteria and use them as “factories” to make the protein.

What is the name of the enzyme that separates the two strands of DNA during replication?

helicase. Helicases are enzymes that bind and may even remodel nucleic acid or nucleic acid protein complexes. There are DNA and RNA helicases. DNA helicases are essential during DNA replication because they separate double-stranded DNA into single strands allowing each strand to be copied.

What is the main job of DNA polymerase?

DNA polymerase is responsible for the process of DNA replication, during which a double-stranded DNA molecule is copied into two identical DNA molecules. Scientists have taken advantage of the power of DNA polymerase molecules to copy DNA molecules in test tubes via polymerase chain reaction, also known as PCR.

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What are the 5 steps of DNA replication in order?

What are the 5 steps of DNA replication in order?

  • Step 1: Replication Fork Formation. Before DNA can be replicated, the double stranded molecule must be “unzipped” into two single strands.
  • Step 2: Primer Binding. The leading strand is the simplest to replicate.
  • Step 3: Elongation.
  • Step 4: Termination.

What kind of process is DNA replication?

DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. Replication is an essential process because, whenever a cell divides, the two new daughter cells must contain the same genetic information, or DNA, as the parent cell.

Which of the following sequences is correct for double stranded DNA?

So, the correct option is ‘ GGCC CCGG ‘.

What is the name of the class of enzymes that recognizes and cuts?

Restriction enzyme: An enzyme from bacteria that can recognize specific base sequences in DNA and cut the DNA at that site (the restriction site). A restriction enzyme acts as a biochemical scissors. Also called a restriction endonuclease.

Which of the statements best describes the way you would engineered bacterial cells to produce a human protein?

The same restriction enzyme would be used to cut both the donor human DNA and the vector DNA. Explanation: The way one could engineer bacterial cells to produce a human protein is by allowing bacteria to taking up the recombinant DNA. This recombinant DNA are inserted in the form of the vectors.

Why do Okazaki fragments form?

Okazaki fragments are formed on the lagging strand for the synthesis of DNA in a 5′ to 3′ direction towards the replication fork. Only one of the two strands of DNA would be replicated in an entity if not for these fragments. This would reduce the efficiency of the process of replication.

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What is DNA complementary strand?

Complementary deoxyribonucleic acid (DNA) is DNA in which the sequence of the constituent molecules on one strand of the double stranded structure chemically matches the sequence on the other strand. In the chemical “lock and key” fit, an A on one strand always pairs with a T on the other strand.

Why does DNA polymerase go from 5 to 3?

DNA replication goes in the 5′ to 3′ direction because DNA polymerase acts on the 3′-OH of the existing strand for adding free nucleotides.

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